Two technologies, one goal: Developing and evaluating anti-Zika virus NS1 antibodies using phage display and hybridoma technologies for diagnostic applications

This study compared phage display and hybridoma technologies for generating antibodies against Zika virus NS1. Phage display-derived antibodies demonstrated greater specificity, while hybridoma-derived antibodies provided greater sensitivity. A combined antibody format achieved a detection limit of 1 ng/mL in spiked human serum and showed no cross-reactivity with dengue-positive serum. Boca Biolistics supplied clinically characterized Zika- and dengue-related serum samples used in the research.

Pantigny R, Combe M, Roesch C, et al. Two technologies, one goal: Developing and evaluating anti-Zika virus NS1 antibodies using phage display and hybridoma technologies for diagnostic applications. Journal of Immunological Methods. 2026;547:114041. doi:10.1016/j.jim.2026.114041.