CDIFF
Roche Cobas 4800 system
(1) automated sample preparation to extract nucleic acids from the unformed stool specimens;(2)PCR amplification of target DNA sequences using C.difficile specific primers, and real-time detection of cleaved fluorescent-labeled C. difficile specific oligonucleotide detection probes.
Collect unformed stool specimen in a sterile container
None
routine venipuncture
draw tube or plastic pour off tube
frozen
if sample is not being tested on the same day of draw, separate and freeze at -20C
Adults: 8.5-72.5 pg/mL
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