Roche Cobas 4800 system
contains two major processes:(1)automated sample preparation to extract nucleic acids from the anogenital lesion specimens;(2)PCR amplification of target DNA sequences using HSV-1 and HSV-2 specific primers, and real-time detection of cleaved fluorescent-labeled HSV-1 and HSV-2 specific oligonucleotide detection probes.
Anogenital lesion swab specimens collected with the Mswab Collection, Transport and Preservation System
ambient or 2-8C
store at 2-8C for up to 96 hours. Specimens in Lysis tubes can be stored at 15-30C for up to 6 days, 2-8C for up to 3 months
Negative: < 15.0 AU/mL Positive: >/= 15.0 AU/mL